Researchers develop an innovative platform for engineering three-dimensional microbial structures while preserving molecular diffusion, microbial motility, and growth

A new liquid-based platform called "floatony" can form and maintain complex three-dimensional microbial structures without solidifying the surrounding medium. Developed by researchers at Institute of Science Tokyo (Science Tokyo) in collaboration with Suntory Global Innovation Center Ltd. (SIC), the platform uses a liquid drawing technique to suspend bacteria within a specially tuned "canvas solution," achieving stable colonies that preserve molecular diffusion, spatial structures, and cell mobility. This approach could offer new ways to study gut microbiota, biofilms, and other spatially organized microbial communities.

World's First Platform for Engineering Three-Dimensional (3D) Microbial Architectures in Liquid

Microbes in the gut, lungs, and biofilms do not simply exist as perfectly mixed populations; instead, they grow in layered and clustered three-dimensional (3D) arrangements. This spatial organization shapes how nutrients and chemical signals move between neighboring cells and how much oxygen can be accessed by different microbes. Scientists increasingly recognize that the relative spatial arrangement of microbes plays a role in digestion, immunity, and overall health. Furthermore, the organization of microbes also influences their community behavior, interspecies interactions, and physiology. In other words, to truly understand microbial communities, it is important to know not just which microbes are present, but where each one is positioned.

Recreating structured microbial communities in the laboratory, however, has proven difficult. Standard culture methods usually grow microbes in well-mixed liquid or on flat agar surfaces, making it hard to capture the 3D organization seen in living systems. More advanced methods can build 3D structures, but they typically rely on hydrogels or other solid materials to hold microbes in place. While these methods can maintain a colony's shape, they can also limit bacterial movement, proliferation, and molecular diffusion, thereby compromising the dynamic liquid environments found in natural microbial communities.

To address this challenge, a research team led by Associate Professor Masayoshi Tanaka from the Department of Chemical Science and Engineering, Institute of Science Tokyo (Science Tokyo), Japan, in collaboration with Suntory Global Innovation Center Ltd., Japan, developed the world's first liquid platform capable of engineering floating 3D microbial architectures, called "floatony."

The study, published in Volume 18, Issue 3 of the journal Biofabrication on June 30, 2026, describes a technique for creating and maintaining complex three-dimensional microbial structures entirely within a liquid, without solidifying the surrounding medium.

The team's approach uses a liquid containing microbes, or "bacterial ink," which is drawn into a "canvas solution" through careful 3D injection with a robotic arm. By carefully tuning the physical properties of the canvas solution, the researchers were able to keep the drawn microbial structures suspended in place without them sinking, floating away, or dispersing. Through rheological measurements, the researchers established practical design principles for liquid environments that simultaneously maintain structural stability and molecular diffusion.

Using Escherichia coli as a model organism, the researchers demonstrated that bacteria in floatonies remained alive and metabolically active. In enzyme activity tests, the bacterial cells were able to process a substrate and release reaction products that diffused outward into the surrounding liquid.

Unlike traditional hydrogel- or microfluidic-based methods, the approach maintains the intrinsic fluidity of the culture medium, allowing microbial motility, diffusion, and self-organization under minimally constrained conditions. This makes it possible to study and engineer microbial spatial organization under conditions that more closely resemble natural liquid environments.

Overall, the findings suggest that floatonies could become a useful experimental platform for studying how spatial organization affects microbial behavior. By bridging the gap between simple liquid cultures and rigid microfluidic- or gel-based systems, floatonies offer a new window into how microbial communities take shape and how their spatial arrangement can be studied and engineered in the laboratory.

The platform could also contribute to a better understanding of how microbial spatial organization influences health and disease, while enabling the rational design of microbial living materials.

“What makes floatony particularly exciting is that it allows us to design three-dimensional microbial communities while preserving a fluid environment in which microbes can move, grow, and interact. We believe this platform could open new possibilities for understanding and designing functional microbial communities, and ultimately for exploring their applications in industrial biotechnology.”

— Ippei Inoue, Suntory Global Innovation Center Ltd.

Reference
Authors: Hidetaka Taniguchi¹, Mai Miyauchi¹, Ippei Inoue²,*, Masayoshi Tanaka¹,*
*Corresponding authors
Title: Floatony formation in liquid environments: Liquid drawing-based fabrication of three-dimensional microbial structures
Journal: Biofabrication
DOI: 10.1088/1758-5090/ae7ed4
Affiliations:
¹ Department of Chemical Science and Engineering, Institute of Science Tokyo, Japan
² Suntory Global Innovation Center Ltd., Japan